刊物名（原文）：Biochemical

Engineering Journal

刊物名（中文）：生物化学工程学报

刊物国别：

发表时间（原文）：2015,101:44–54

发表时间（中文）：发表于2015年101期44-54页

刊物类型（EI/SCI/SSCI），级别：SCI影响因子2.45分类二区

论文题目（原文）：Rhamnolipid production

from waste cooking oil using Pseudomonas SWP-4

论文题目（中文）：Pseudomonas SWP-4菌株转化餐厨废油生产鼠李糖脂

论文作者（原文）：Guihong Lan, Qiang Fan, Yongqiang Liu, Chao

Chen，Guixiang Li , Yu Liu, Xiaobo Yin

论文作者（中文）：兰贵红，范强，刘永强，陈超，李桂香，刘余，尹小波

论文摘要（原文）：The present work aims to produce rhamnolipid from waste cooking oil (WCO) using a newly isolated bacterium named Pseudomonas SWP-4. SWP-4 was a high-yield strain that could accumulate rhamnolipid steadily even in decline phase and gave a maximum rhamnolipid yield of 13.93 g/L and WCO utilization percent around 88%. The critical micelle concentration of the produced rhamnolipid was only 27 mg/L and its emulsification index against n-hexadecane reached around 59%. Moreover, it reduced the surface tension of water from 71.8 mN/m to 24.1 mN/m and the interfacial tension against n-hexadecane from 29.4 mN/m to 0.9 mN/m. Results of biosurfactant stability show the rhamnolipid was effective when the salinity was lower than 8% and pH value ranged from 4 to 10, and it was quite thermostable based on thermal gravity analysis. Furthermore, it maintained high surface activity even after incubation under extreme conditions i.e., pH of 4.0, salinity of 8% and temperature of 80 ?C for half a month. Based on free fatty acids metabolism analysis, Pseudomonas SWP-4 consumed palmitic acid, oleic acid and linoleic acid chiefly. All these characteristics demonstrate bioconversion and biodegradation of WCO by Pseudomonas SWP-4 is a promising and commercial way of rhamnolipid production and waste treatment.

论文摘要（中文）：本研究利用Pseudomonas SWP-4菌株转化餐厨废油生产鼠李糖脂，优化后其最佳产率为13.93g/L，餐厨废油的转化率为88%；产物的临界胶束浓度(CMC)为27mg/L,乳化n-十六烷乳化率为59%。对水体的表面张力从71.8mN/m降低至24.1mN/m。结果显示产物有广泛的耐盐性，耐温性，基本不受温度、pH值与盐浓度影响。

对应开放实验题名：生物转化餐厨废油产鼠李糖脂假单胞菌株的分离与代谢机理

对应开放实验编号：KSZ14094

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